Chemi-Verse(tm) HPK1 (MAP4K1) Kinase Assay Kit

BPS Bioscience

Chemi-Verse(tm) HPK1 (MAP4K1) Kinase Assay Kit

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Research Use Only

The Chemi-Verse™ HPK1 Kinase Assay Kit is designed to measure HPK1 (Hematopoietic progenitor kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified HPK1 (amino acids 1-346, kinase domain), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions. Note: MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a universal substrate for many in vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

See manual

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