MPLA from S. minnesota R595 (Re) TLRpure Sterile Solution

Catalog number: IAX-100-002-C250
Brand: Innaxon
Packing: 250 ug
Other sizes: Other sizes available
Price: € 132.00
Expected delivery time: 7 days
Quantity:

Product specifications for - MPLA from S. minnesota R595 (Re) TLRpure Sterile Solution

Overview: 
Product group: Chemicals
Category: Other
Properties: 
Purity: >99.9%. No detectable DNA, RNA and protein traces.
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352200
Concentration: 1 mg/ml
Form supplied: Liquid. Colourless opaque aqueous solution.
Storage instructions: 2-¦C to 8-¦C
Scientific information: 
Scientific info: Activation of cells by LPS is mediated by the Toll-like receptor 4 (TLR4), a member of the highly conserved protein family of TLRs, which are specialised in the recognition of microbial components. In mice, defects in TLR4 result in LPS unresponsiveness. For optimal interaction with LPS, TLR4 requires association with myeloid differentiation protein 2 (MD-2). According to current consensus activation of TLR4 is preceded by the transfer of LPS to membrane-bound (m) or soluble (s) CD14 by LPS-binding protein (LBP). This mechanism is believed to be generally true for LPS signaling. Re-form LPS and lipid A, but not S-form LPS, are capable of inducing TNF-alpha responses also in the absence of CD14. LPS, synthesized by most wild-type (WT) Gram-negative bacteria (S-form LPS), consists of three regions, the O-polysaccharide chain, which is made up of repeating oligosaccharide units, the core oligosaccharide and the lipid A, which harbors the endotoxic activity of the entire molecule. R-form LPS synthesized by the so-called rough (R) mutants of Gram-negative bacteria lacks the O-specific chain. Furthermore, the core-oligosaccharide may be present in different degrees of completion, depending on the class (Ra to Re) to which the mutant belongs. Monophosphoryl Lipid A (MPLA) represents a detoxified derivative of Lipid A and constitutes an important adjuvant in prophylactic and therapeutic vaccines. - Chemical. Isolated and purified from S. minnesota strain R595. Activation of cells by LPS is mediated by the Toll-like receptor 4 (TLR4), a member of the highly conserved protein family of TLRs, which are specialised in the recognition of microbial components. In mice, defects in TLR4 result in LPS unresponsiveness. For optimal interaction with LPS, TLR4 requires association with myeloid differentiation protein 2 (MD-2). According to current consensus activation of TLR4 is preceded by the transfer of LPS to membrane-bound (m) or soluble (s) CD14 by LPS-binding protein (LBP). This mechanism is believed to be generally true for LPS signaling. Re-form LPS and lipid A, but not S-form LPS, are capable of inducing TNF-alpha responses also in the absence of CD14. LPS, synthesized by most wild-type (WT) Gram-negative bacteria (S-form LPS), consists of three regions, the O-polysaccharide chain, which is made up of repeating oligosaccharide units, the core oligosaccharide and the lipid A, which harbors the endotoxic activity of the entire molecule. R-form LPS synthesized by the so-called rough (R) mutants of Gram-negative bacteria lacks the O-specific chain. Furthermore, the core-oligosaccharide may be present in different degrees of completion, depending on the class (Ra to Re) to which the mutant belongs. Monophosphoryl Lipid A (MPLA) represents a detoxified derivative of Lipid A and constitutes an important adjuvant in prophylactic and therapeutic vaccines.
Safety information: 
MSDS: MSDS
Hazard information: Non-hazardous, Warning
Additional information: 
Synonyms: IAX-100-002-C250; Innaxon
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Read more
Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 24, 116 (1971) Read more
Endotoxic properties of chemically synthesized lipid A part structures. Comparison of synthetic lipid A precursor and synthetic analogues with biosynthetic lipid A precursor and free lipid A: C. Galanos, et al.; Eur. J. Biochem. 140, 221 (1984) Read more
Biological activities of synthetic lipid A analogs: pyrogenicity, lethal toxicity, anticomplement activity, and induction of gelation of Limulus amoebocytelysate: K. Tanamoto, et al.; Infect. Immun. 44, 421 (1984) Read more
Biological activity of synthetic heptaacyl lipid A representing a component of Salmonella minnesota R595 lipid A: C. Galanos, et al.; Eur. J. Biochem. 160, 55 (1986) Read more