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Chemokine (C-C motif) ligand 6 (CCL6) Polyclonal Antibody

CAU26590
Biomatik
ApplicationsWestern Blot
Product group Antibodies
TargetCcl6
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Overview

  • Supplier
    Biomatik
  • Product Name
    Chemokine (C-C motif) ligand 6 (CCL6) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID20305
  • Target name
    Ccl6
  • Target description
    C-C motif chemokine ligand 6
  • Target synonyms
    MRP-1, Scya6, c10, C-C motif chemokine 6, CC chemokine C10, chemokine (C-C motif) ligand 6, small-inducible cytokine A6
  • Host
    Rabbit
  • Protein IDP27784
  • Protein Name
    C-C motif chemokine 6
  • Scientific Description
    The Chemokine (C-C motif) ligand 6 (CCL6) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL6 using anti-CCL6 antibody (A30384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CCL6 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL6 antigen affinity purified polyclonal antibody (Catalog # A30384) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL6 at approximately 11KD. The expected band size for CCL6 is at 11KD.
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