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ProductsBack/CHI3L1 / YKL-40 Antibody (Internal)

CHI3L1 / YKL-40 Antibody (Internal)

LS-C354504
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCHI3L1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CHI3L1 / YKL-40 Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1116
  • Target name
    CHI3L1
  • Target description
    chitinase 3 like 1
  • Target synonyms
    ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39, chitinase-3-like protein 1, 39 kDa synovial protein, cartilage glycoprotein 39, chitinase 3-like 1 (cartilage glycoprotein-39)
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHI3L1 using anti-CHI3L1 antibody (A01283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHI3L1 antigen affinity purified polyclonal antibody (Catalog # A01283-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHI3L1 at approximately 43KD. The expected band size for CHI3L1 is at 43KD.
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