Chicken DAO(Diamine Oxidase) ELISA Kit

Biorbyt

Chicken DAO(Diamine Oxidase) ELISA Kit

10

standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken DAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken DAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chicken DAO in the samples is then determined by comparing the OD of the samples to the standard curve

0.63-40 ng/mL

0.241 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diamine Oxidase(DAO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diamine Oxidase(DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diamine Oxidase(DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Diamine Oxidase(DAO) in the samples is then determined by comparing the OD of the samples to the standard curve.

Chicken

41116158