Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
Chicken IgG (H&L) Antibody Peroxidase Conjugated
TA397603
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
Overview
- SupplierOriGene
- Product NameChicken IgG (H&L) Antibody Peroxidase Conjugated
- Delivery Days Customer14
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- HostRabbit
- Scientific DescriptionChicken IgG (H&L) Antibody Peroxidase Conjugated
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203