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Chk2 antibody [8F12]

Research Use Only
GTX70295
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCHEK2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Chk2 antibody [8F12]
  • Delivery Days Customer
    9
  • Antibody Specificity
    GTX70295 is specific for human CHK2 protein.
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8F12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Formulation
    Liquid
  • Gene ID11200
  • Target name
    CHEK2
  • Target description
    checkpoint kinase 2
  • Target synonyms
    CDS1; cds1 homolog; checkpoint-like protein CHK2; CHK2; CHK2 checkpoint homolog; hCds1; HuCds1; LFS2; PP1425; RAD53; serine/threonine-protein kinase Chk2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO96017
  • Protein Name
    Serine/threonine-protein kinase Chk2
  • Scientific Description
    In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Repair of DNA damage induced by the novel nucleoside analogue CNDAG through homologous recombination. Liu X et al., 2020 Apr, Cancer Chemother Pharmacol
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  • ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress. Park SH et al., 2019 Dec 16, Nat Commun
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  • Homeodomain Proteins Directly Regulate ATM Kinase Activity. Johnson TE et al., 2018 Aug 7, Cell Rep
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  • ATM directs DNA damage responses and proteostasis via genetically separable pathways. Lee JH et al., 2018 Jan 9, Sci Signal
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  • Direct activation of ATM by resveratrol under oxidizing conditions. Lee JH et al., 2014, PLoS One
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  • A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species. Chen HM et al., 2011 May 1, Free Radic Biol Med
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