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Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CHEK2 Polyclonal Antibody

BS-1391R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCHEK2
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Overview

  • Supplier
    Bioss
  • Product Name
    CHEK2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID11200
  • Target name
    CHEK2
  • Target description
    checkpoint kinase 2
  • Target synonyms
    CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, TPDS4, hCds1, serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, cds1 homolog, checkpoint-like protein CHK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96017
  • Protein Name
    Serine/threonine-protein kinase Chk2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang L, Wang QT, Liu YP, et al. ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells. J Gastric Cancer. 2017,17(4):295-305. doi: 10.5230/jgc.2017.17.e33
    Read this paper
  • Liu J, Yang M, Jing L, et al. Silica nanoparticle exposure inducing granulosa cell apoptosis and follicular atresia in female Balb/c mice. Environ Sci Pollut Res Int. 2018,25(4):3423-3434. doi: 10.1007/s11356-017-0724-5
    Read this paper
  • Wei J, Zhang L, Ren L, et al. Endosulfan induces cell dysfunction through cycle arrest resulting from DNA damage and DNA damage response signaling pathways. Sci Total Environ. 2017,589:97-106. doi: 10.1016/j.scitotenv.2017.02.154
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CHEK2 using anti-CHEK2 antibody (A00277-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 antigen affinity purified polyclonal antibody (Catalog # A00277-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2 at approximately 62 kDa. The expected band size for CHEK2 is at 61 kDa.
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