
Sample(30μg whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: HeLa S3 (GTX14654) E: Hep G2 (GTX27900) 12% SDS PAGE GTX106692 diluted at 1:1000
CHMP5 antibody [N1C3]
GTX106692
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCHMP5
Overview
- SupplierGeneTex
- Product NameCHMP5 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51510
- Target nameCHMP5
- Target descriptioncharged multivesicular body protein 5
- Target synonymsC9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, Spike, Vps60, charged multivesicular body protein 5, SNF7 domain containing 2, SNF7 domain-containing protein 2, apoptosis-related protein PNAS-2, chromatin-modifying protein 5, hVps60, small protein with inherent killing effect, vacuolar protein sorting-associated protein 60
- HostRabbit
- IsotypeIgG
- Protein IDQ9NZZ3
- Protein NameCharged multivesicular body protein 5
- Scientific DescriptionCHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500. Blue: Hoechst 33342 staining. CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX106692/GTX106692_41066_IFA_w_23060120_412.webp)
![Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Jurkat whole cell extracts and Jurkat exosome extract (15.4 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX106692/GTX106692_40002_20190607_WB_Fraction_w_23060120_331.webp)

![CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX106692/GTX106692_41066_20131127_IHC-P_M_w_23060120_535.webp)





