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ChoosE3-Freedom(tm) Intrachain TR-FRET Assay Kit

ChoosE3-Freedom(tm) Intrachain TR-FRET Assay Kit

Research Use Only
78560
BPS Bioscience
Product group Assays
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More than 130 suppliers
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    ChoosE3-Freedom(tm) Intrachain TR-FRET Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The ChoosE3-Freedom Intrachain TR-FRET Assay Kit contains E1 and E2 enzymes, ATP, an optimized TRF Ubiquitin Mix, and a universal buffer. Purified E3 ligase MDM2 is also provided as an internal quality control. The assay was designed with a Europium-labeled Ubiquitin donor and a Cy5-labeled Ubiquitin acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on the E3 ligase, the assay measures only poly-ubiquitination and not mono-ubiquitination. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics.
  • Storage Instruction
    See manual
  • UNSPSC
    41116133