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Western Blot analysis of Jurkat cells using CHP2 Polyclonal Antibody
Western Blot analysis of Jurkat cells using CHP2 Polyclonal Antibody
Western Blot analysis of Jurkat cells using CHP2 Polyclonal Antibody

CHP2 Antibody

CSB-PA001642
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63928
  • Target name
    CHP2
  • Target description
    calcineurin like EF-hand protein 2
  • Target synonyms
    calcineurin B homologous protein 2, hepatocellular carcinoma antigen gene 520, hepatocellular carcinoma-associated antigen 520
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43745
  • Protein Name
    Calcineurin B homologous protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHP2 using anti-CHP2 antibody (A08478-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHP2 antigen affinity purified polyclonal antibody (A08478-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHP2 at approximately 22 kDa. The expected band size for CHP2 is at 22 kDa.
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