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ProductsBack/CHRM2 / M2 Antibody

CHRM2 / M2 Antibody

LS-C331546
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCHRM2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CHRM2 / M2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 51kDa, while the observed MW by Western blot was 52kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 51kDa, while the observed MW by Western blot was 52kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1129
  • Target name
    CHRM2
  • Target description
    cholinergic receptor muscarinic 2
  • Target synonyms
    7TM receptor; acetylcholine receptor, muscarinic 2; HM2; muscarinic acetylcholine receptor M2; muscarinic M2 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHRM2 using anti-CHRM2 antibody (A02733). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat brain tissue lysates, lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM2 antigen affinity purified polyclonal antibody (Catalog # A02733) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRM2 at approximately 52KD. The expected band size for CHRM2 is at 52KD.
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