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SH-SY-5Y whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Chromogranin A antibody (GTX113165) diluted at 1:500 and competitor's antibody (sc-1488) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SH-SY-5Y whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Chromogranin A antibody (GTX113165) diluted at 1:500 and competitor's antibody (sc-1488) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SH-SY-5Y whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Chromogranin A antibody (GTX113165) diluted at 1:500 and competitor's antibody (sc-1488) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Chromogranin A antibody

GTX113165
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCHGA
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Overview

  • Supplier
    GeneTex
  • Product Name
    Chromogranin A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.22 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1113
  • Target name
    CHGA
  • Target description
    chromogranin A
  • Target synonyms
    CGA, PHE5, PHES, chromogranin-A, SP-I, betagranin (N-terminal fragment of chromogranin A), catestatin, chromofungin, parathyroid secretory protein 1, pituitary secretory protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10645
  • Protein Name
    Chromogranin-A
  • Scientific Description
    The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu SY, Wu HT, Wang YC, et al. Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo. Autophagy. 2023,19(4):1239-1257. doi: 10.1080/15548627.2022.2123098
    Read this paper
  • Song F, Wang S, Pang X, et al. An Active Fraction of Trillium tschonoskii Promotes the Regeneration of Intestinal Epithelial Cells After Irradiation. Front Cell Dev Biol. 2021,9:745412. doi: 10.3389/fcell.2021.745412
    Read this paper
  • Wang S, Han Y, Zhang J, et al. Me6TREN targets β-catenin signaling to stimulate intestinal stem cell regeneration after radiation. Theranostics. 2020,10(22):10171-10185. doi: 10.7150/thno.46415
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.
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