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Western blot All lanes: CHTOP antibody at 14 ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 22 kDa Observed band size: 27, 52 kDa
Western blot All lanes: CHTOP antibody at 14 ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 22 kDa Observed band size: 27, 52 kDa
Western blot All lanes: CHTOP antibody at 14 ug/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 22 kDa Observed band size: 27, 52 kDa

CHTOP Antibody

CSB-PA897109HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCHTOP
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHTOP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26097
  • Target name
    CHTOP
  • Target description
    chromatin target of PRMT1
  • Target synonyms
    C10orf77; C1orf77; chromatin target of PRMT1 protein; FL-SRAG; FOP; friend of PRMT1 protein; pp7704; small protein rich in arginine and glycine; SRAG; SRAG-3; SRAG-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3Y2
  • Protein Name
    Chromatin target of PRMT1 protein
  • Scientific Description
    Plays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse spleen tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf77/FOP/CHTOP antigen affinity purified polyclonal antibody (Catalog # A07770-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1orf77/FOP/CHTOP at approximately 28 kDa. The expected band size for C1orf77/FOP/CHTOP is at 26 kDa.
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