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Chymotrypsin C Antibody (aa30-268)

LS-C372699
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCTRC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Chymotrypsin C Antibody (aa30-268)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:50 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11330
  • Target name
    CTRC
  • Target description
    chymotrypsin C
  • Target synonyms
    caldecrin; chymotrypsin-C; chymotrypsinogen C; CLCR; ELA4; elastase 4; elastase IV; serum calcium decreasing factor
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTRC using anti-CTRC antibody (A03540-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTRC antigen affinity purified polyclonal antibody (A03540-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTRC at approximately 34 kDa. The expected band size for CTRC is at 29 kDa.
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