Citrate synthase antibody [N2C3] detects Citrate synthase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_44664_20220708_IHC-P_M_22071823_202.webp "Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Citrate synthase antibody [N2C3] detects Citrate synthase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. Citrate synthase antibody [N2C3] (GTX110624) dilution: 1:50.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_41773_20151008_IHC-Wm_Z_22111423_697.webp "Citrate synthase antibody [N2C3] detects Citrate synthase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. Citrate synthase antibody [N2C3] (GTX110624) dilution: 1:50.")
![Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_43677_20190913_WB_Z_22111423_856.webp "Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Citrate synthase antibody [N2C3] detects Citrate synthase protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo. Green: Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_43250_20180703_IHC-WM_Z_22111423_838.webp "Citrate synthase antibody [N2C3] detects Citrate synthase protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo. Green: Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.")
![Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_43901_20200605_IHC-P_M_w_23060500_625.webp "Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] (GTX110624). Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_41724_20150209_IP_w_23060500_878.webp "Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] (GTX110624). Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_41969_20150409_IHC-P_M_2_w_23060500_179.webp "Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110624/GTX110624_43901_20200605_IHC-P_R_w_23060500_815.webp "Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Citrate synthase antibody [N2C3] detects Citrate synthase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Citrate synthase antibody [N2C3]
GTX110624
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Chicken, Human, Monkey, Mouse, Rat, Zebra Fish
TargetCS
Overview
- SupplierGeneTex
- Product NameCitrate synthase antibody [N2C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.65 mg/ml
- ConjugateUnconjugated
- Gene ID1431
- Target nameCS
- Target descriptioncitrate synthase
- Target synonymscitrate synthase, mitochondrial, citrate (Si)-synthase
- HostRabbit
- IsotypeIgG
- Protein IDO75390
- Protein NameCitrate synthase, mitochondrial
- Scientific DescriptionThe protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]
- ReactivityAmphibian, Chicken, Human, Monkey, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Todorova V, Stauffacher MF, Ravotto L, et al. Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation. Mol Neurodegener. 2023,18(1):15. doi: 10.1186/s13024-023-00602-xRead this paper
- Demchenko IT, Suliman HB, Zhilyaey SY, et al. GAT inhibition preserves cerebral blood flow and reduces oxidant damage to mitochondria in rodents exposed to extreme hyperbaric oxygen. Front Mol Neurosci. 2022,15:1062410. doi: 10.3389/fnmol.2022.1062410Read this paper
- Todorova V, Merolla L, Karademir D, et al. Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina. Sci Rep. 2022,12(1):20195. doi: 10.1038/s41598-022-24586-8Read this paper
- Lin CC, Yan J, Kapur MD, et al. Parkin coordinates mitochondrial lipid remodeling to execute mitophagy. EMBO Rep. 2022,23(12):e55191. doi: 10.15252/embr.202255191Read this paper
- Zittlau KI, Lechado-Terradas A, Nalpas N, et al. Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy. Mol Cell Proteomics. 2022,21(2):100191. doi: 10.1016/j.mcpro.2021.100191Read this paper
- Suliman H, Ma Q, Zhang Z, et al. Annexin A1 Tripeptide Mimetic Increases Sirtuin-3 and Augments Mitochondrial Function to Limit Ischemic Kidney Injury. Front Physiol. 2021,12:683098. doi: 10.3389/fphys.2021.683098Read this paper
- Al-Attar R, Storey KB. RAGE against the stress: Mitochondrial suppression in hypometabolic hearts. Gene. 2020,761:145039. doi: 10.1016/j.gene.2020.145039Read this paper
- Ding CC, Rose J, Sun T, et al. MESH1 is a cytosolic NADPH phosphatase that regulates ferroptosis. Nat Metab. 2020,2(3):270-277. doi: 10.1038/s42255-020-0181-1Read this paper
- Leandro J, Dodatko T, Aten J, et al. DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. Hum Mol Genet. 2020,29(7):1168-1179. doi: 10.1093/hmg/ddaa037Read this paper
- Leandro J, Violante S, Argmann CA, et al. Mild inborn errors of metabolism in commonly used inbred mouse strains. Mol Genet Metab. 2019,126(4):388-396. doi: 10.1016/j.ymgme.2019.01.021Read this paper
Datasheet
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