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ProductsBack/CKII alpha antibody [N1C3]

CKII alpha antibody [N1C3]

GTX110726
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCSNK2A2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CKII alpha antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1459
  • Target name
    CSNK2A2
  • Target description
    casein kinase 2 alpha 2
  • Target synonyms
    casein kinase 2 alpha'; casein kinase 2, alpha prime polypeptide; casein kinase II subunit alpha'; CK II alpha'; CK2A2; CK2alpha'; CSNK2A1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19784
  • Protein Name
    Casein kinase II subunit alpha'
  • Scientific Description
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates Ser-392 of p53/TP53 following UV irradiation.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CSNK2A2 using anti-CSNK2A2 antibody (A04722-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2A2 antigen affinity purified polyclonal antibody (Catalog # A04722-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK2A2 at approximately 39 kDa. The expected band size for CSNK2A2 is at 41 kDa.
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