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Claudin 11 Antibody (YA2334, Rabbit)

HY-P82589
MedChem Express
TargetCLDN11
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Claudin 11 Antibody (YA2334, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5010
  • Target name
    CLDN11
  • Target description
    claudin 11
  • Target synonyms
    claudin-11; HLD22; oligodendrocyte transmembrane protein; oligodendrocyte-specific protein; OSP; OTM
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Claudin 11 Antibody (YA2334) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2334), targeting Claudin 11, with a predicted molecular weight of 22 kDa (observed band size: 22 kDa). Claudin 11 Antibody (YA2334) can be used for WB, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CLDN11 using anti-CLDN11 antibody (A08381-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLDN11 antigen affinity purified polyclonal antibody (Catalog # A08381-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLDN11 at approximately 22 kDa. The expected band size for CLDN11 is at 22 kDa.
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