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Paraformaldehyde-fixed, paraffin embedded Human gall bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Claudin 2 Monoclonal Antibody, Unconjugated (bsm-33414M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gall bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Claudin 2 Monoclonal Antibody, Unconjugated (bsm-33414M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gall bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Claudin 2 Monoclonal Antibody, Unconjugated (bsm-33414M) at 1:200 overnight at 4°C, DAB staining.

Claudin 2 Monoclonal Antibody

BSM-33414M
Bioss Antibodies
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCLDN2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Claudin 2 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10G2
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9075
  • Target name
    CLDN2
  • Target description
    claudin 2
  • Target synonyms
    claudin-2; OAZON; SP82
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57739
  • Protein Name
    Claudin-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Claudin 2/CLDN2 using anti-Claudin 2/CLDN2 antibody (A03033-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 2/CLDN2 antigen affinity purified polyclonal antibody (Catalog # A03033-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 2/CLDN2 at approximately 23 kDa. The expected band size for Claudin 2/CLDN2 is at 23 kDa.
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