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Claudin 3 antibody

GTX135322
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCLDN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Claudin 3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.78 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1365
  • Target name
    CLDN3
  • Target description
    claudin 3
  • Target synonyms
    C7orf1; claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-R2; CPE-receptor 2; CPETR2; HRVP1; RVP1; ventral prostate.1 protein homolog; ventral prostate.1-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15551
  • Protein Name
    Claudin-3
  • Scientific Description
    Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 3/CLDN3 antigen affinity purified polyclonal antibody (Catalog # A04393-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 23KD. The expected band size for Claudin 3/CLDN3 is at 23KD.
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