![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1528] (GTX637001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1528] (GTX637001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637001/GTX637001_44732_20220708_WB_22071401_940.webp)
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1528] (GTX637001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Claudin 3 antibody [HL1528]
GTX637001
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCLDN3
Overview
- SupplierGeneTex
- Product NameClaudin 3 antibody [HL1528]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1528
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1365
- Target nameCLDN3
- Target descriptionclaudin 3
- Target synonymsC7orf1, CPE-R2, CPETR2, HRVP1, RVP1, claudin-3, CPE-R 2, CPE-receptor 2, Clostridium perfringens enterotoxin receptor 2, ventral prostate.1 protein homolog, ventral prostate.1-like protein
- HostRabbit
- IsotypeIgG
- Protein IDO15551
- Protein NameClaudin-3
- Scientific DescriptionTight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Claudin 3 antibody [HL1528] detects Claudin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1528] (GTX637001) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Claudin 3 antibody [HL1528] detects Claudin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1528] (GTX637001) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637001/GTX637001_44732_20221230_ICC_IF_22122901_404.webp)





![Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Claudin 3 stained by Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637000/GTX637000_T-44683_20220617_IHC-P_22062121_662.webp)
![IHC-P analysis of human fallopian tube mucosa tissue using GTX639933 Claudin 3 antibody [HMV309] HistoMAX?. Strong membranous claudin 3 staining of epithelial cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639933/GTX639933_20240514_IHC-P_24051319_182.webp)