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WB analysis of human fetal lung tissue using GTX47766 Claudin 7 antibody at 0.2-1microg/ml.
WB analysis of human fetal lung tissue using GTX47766 Claudin 7 antibody at 0.2-1microg/ml.
WB analysis of human fetal lung tissue using GTX47766 Claudin 7 antibody at 0.2-1microg/ml.

Claudin 7 antibody, Internal

GTX47766
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCLDN7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Claudin 7 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1366
  • Target name
    CLDN7
  • Target description
    claudin 7
  • Target synonyms
    CEPTRL2; claudin-1; claudin-7; CLDN-7; clostridium perfringens enterotoxin receptor-like 2; CPETRL2; epididymis secretory sperm binding protein; Hs.84359
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95471
  • Protein Name
    Claudin-7
  • Scientific Description
    This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Claudin 7/CLDN-7 using anti-Claudin 7/CLDN-7 antibody (A03851-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 7/CLDN-7 antigen affinity purified polyclonal antibody (Catalog # A03851-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 7/CLDN-7 at approximately 22 kDa. The expected band size for Claudin 7/CLDN-7 is at 22 kDa.
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