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ClC-5 / CLCN5 Antibody

LS-C334230
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCLCN5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ClC-5 / CLCN5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 83kDa/90kDa, while the observed MW by Western blot was 100kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:20 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 83kDa/90kDa, while the observed MW by Western blot was 100kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.96 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1184
  • Target name
    CLCN5
  • Target description
    chloride voltage-gated channel 5
  • Target synonyms
    CLC5, CLCK2, ClC-5, DENT1, DENTS, NPHL1, NPHL2, XLRH, XRN, hCIC-K2, H(+)/Cl(-) exchange transporter 5, chloride channel, voltage-sensitive 5, chloride transporter ClC-5, voltage-gated chloride ion channel CLCN5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ClC-5/CLCN5 using anti-ClC-5/CLCN5 antibody (A02286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ClC-5/CLCN5 antigen affinity purified polyclonal antibody (Catalog # A02286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ClC-5/CLCN5 at approximately 100 kDa. The expected band size for ClC-5/CLCN5 is at 91 kDa.
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