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CLIC1 Antibody (YA2807, Rabbit)

HY-P83062
MedChem Express
TargetCLIC1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CLIC1 Antibody (YA2807, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1192
  • Target name
    CLIC1
  • Target description
    chloride intracellular channel 1
  • Target synonyms
    CL1C1, CLCNL1, G6, NCC27, chloride intracellular channel protein 1, RNCC protein, chloride channel ABP, glutaredoxin-like oxidoreductase CLIC1, glutathione-dependent dehydroascorbate reductase CLIC1, hRNCC, nuclear chloride ion channel 27, nuclear chloride ion channel protein, p64CLCP, regulatory nuclear chloride ion channel protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CLIC1 Antibody (YA2807) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2807), targeting CLIC1, with a predicted molecular weight of 27 kDa (observed band size: 27 kDa). CLIC1 Antibody (YA2807) can be used for WB, ICC/IF, IP experiment in human, mouse, rat, hamster background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CLIC1 using anti-CLIC1 antibody (A02397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U-937 whole cell lysates, Lane 7: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC1 antigen affinity purified polyclonal antibody (Catalog # A02397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIC1 at approximately 32 kDa. The expected band size for CLIC1 is at 32 kDa.
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