Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads
TA150037
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
Product group Antibodies
Overview
- SupplierOriGene
- Product NameClone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads
- Delivery Days Customer14
- ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDOTI4C5 (formerly 4C5)
- ConjugateAgarose Beads, Agarose
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionClone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads
- UNSPSC12352203