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Western blot All lanes: CLPB antibody at 5ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 79, 76, 73, 75, 58 kDa Observed band size: 79 kDa
Western blot All lanes: CLPB antibody at 5ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 79, 76, 73, 75, 58 kDa Observed band size: 79 kDa
Western blot All lanes: CLPB antibody at 5ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 79, 76, 73, 75, 58 kDa Observed band size: 79 kDa

CLPB Antibody

CSB-PA884428LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCLPB
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLPB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81570
  • Target name
    CLPB
  • Target description
    caseinolytic mitochondrial matrix peptidase chaperone subunit B
  • Target synonyms
    ANKCLB; ankyrin-repeat containing bacterial clp fusion; caseinolytic peptidase B protein homolog; ClpB caseinolytic peptidase B homolog; ClpB homolog, mitochondrial AAA ATPase chaperonin; HSP78; MEGCANN; MGCA7; SKD3; suppressor of potassium transport defect 3; testicular secretory protein Li 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H078
  • Protein Name
    Caseinolytic peptidase B protein homolog
  • Scientific Description
    May function as a regulatory ATPase and be related to secretion/protein trafficking process.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CLPB using anti-CLPB antibody (A02912-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: huamn SW620 whole cell lysates, Lane 3: huamn 22RV1 whole cell lysates, Lane 4: huamn THP-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPB antigen affinity purified polyclonal antibody (Catalog # A02912-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPB at approximately 70KD. The expected band size for CLPB is at 70KD.
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