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ProductsBack/CLPP antibody [C2C3], C-term

CLPP antibody [C2C3], C-term

GTX104656
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetCLPP
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Overview

  • Supplier
    GeneTex
  • Product Name
    CLPP antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8192
  • Target name
    CLPP
  • Target description
    caseinolytic mitochondrial matrix peptidase proteolytic subunit
  • Target synonyms
    ATP-dependent Clp protease proteolytic subunit, mitochondrial; ATP-dependent protease ClpAP, proteolytic subunit, human; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; DFNB81; endopeptidase Clp; PRLTS3; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16740
  • Protein Name
    ATP-dependent Clp protease proteolytic subunit, mitochondrial
  • Scientific Description
    The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Senescent Hepatocytes in Decompensated Liver Show Reduced UPRMT and Its Key Player, CLPP, Attenuates Senescence In Vitro. Sen B et al., 2019, Cell Mol Gastroenterol Hepatol
    Read more

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Figure 1. Western blot analysis of CLPP using anti-CLPP antibody (A01117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPP antigen affinity purified polyclonal antibody (Catalog # A01117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPP at approximately 26KD. The expected band size for CLPP is at 26KD.
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