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WB analysis of human heart tissue lysate using GTX53477 CLPX antibody. Loading : 35ug per lane Dilution : 1:1000
WB analysis of human heart tissue lysate using GTX53477 CLPX antibody. Loading : 35ug per lane Dilution : 1:1000
WB analysis of human heart tissue lysate using GTX53477 CLPX antibody. Loading : 35ug per lane Dilution : 1:1000

CLPX antibody

GTX53477
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCLPX
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Overview

  • Supplier
    GeneTex
  • Product Name
    CLPX antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:50-1:100. FACS: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10845
  • Target name
    CLPX
  • Target description
    caseinolytic mitochondrial matrix peptidase chaperone subunit X
  • Target synonyms
    ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; ClpX caseinolytic peptidase X homolog; ClpX caseinolytic protease X homolog; energy-dependent regulator of proteolysis; EPP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76031
  • Protein Name
    ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
  • Scientific Description
    The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Nov 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CLPX using anti-CLPX antibody (A00978-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPX antigen affinity purified polyclonal antibody (Catalog # A00978-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPX at approximately 69KD. The expected band size for CLPX is at 69KD.
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