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Western Blot analysis of Hela cells using Clusterin Polyclonal Antibody
Western Blot analysis of Hela cells using Clusterin Polyclonal Antibody
Western Blot analysis of Hela cells using Clusterin Polyclonal Antibody

CLU Antibody

CSB-PA001725
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCLU
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLU Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1191
  • Target name
    CLU
  • Target description
    clusterin
  • Target synonyms
    AAG4, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, clusterin, aging-associated protein 4, apolipoprotein J, complement cytolysis inhibitor, complement lysis inhibitor, complement-associated protein SP-40,40, epididymis secretory sperm binding protein, ku70-binding protein 1, sulfated glycoprotein 2, testosterone-repressed prostate message 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10909
  • Protein Name
    Clusterin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clusterin/CLU antigen affinity purified polyclonal antibody (Catalog # A00590-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clusterin/CLU at approximately 70 kDa. The expected band size for Clusterin/CLU is at 52 kDa.
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