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CNPase Antibody

LS-C123271
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCNP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CNPase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues. Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells. Western Blot: 1:2000-1:5000.. ICC (1:1000 - 1:2000), IHC (1:1000 - 1:2000), WB (1:2000 - 1:5000) Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues. Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells. Western Blot: 1:2000-1:5000.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1267
  • Target name
    CNP
  • Target description
    2',3'-cyclic nucleotide 3' phosphodiesterase
  • Target synonyms
    2', 3' cyclic nucleotide 3' phosphohydrolase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNP1; CNPase; HLD20
  • Host
    Chicken
  • Isotype
    IgY
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysate, Lane 7: mouse brain tissue lysate, Lane 8: mouse Neuro-2a whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNPase/CNP antigen affinity purified polyclonal antibody (Catalog # A01017-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNPase/CNP at approximately 45 kDa. The expected band size for CNPase/CNP is at 45 kDa.
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