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WB analysis of various sample lysates using GTX66337 CNTRL antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66337 CNTRL antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66337 CNTRL antibody. Dilution : 1:1000 Loading : 25microg per lane

CNTRL antibody

GTX66337
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCNTRL
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Overview

  • Supplier
    GeneTex
  • Product Name
    CNTRL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11064
  • Target name
    CNTRL
  • Target description
    centriolin
  • Target synonyms
    CEP1, CEP110, FAN, bA165P4.1, centriolin, 110 kDa centrosomal protein, bA165P4.1 (ortholog of mouse Ma2a8), bA165P4.2 (centrosomal protein 1), centriole associated protein, centrosomal protein 110kDa, centrosomal protein of 110 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z7A1
  • Protein Name
    Centriolin
  • Scientific Description
    This gene encodes a centrosomal protein required for the centrosome to function as a microtubule organizing center. The gene product is also associated with centrosome maturation. One version of stem cell myeloproliferative disorder is the result of a reciprocal translocation between chromosomes 8 and 9, with the breakpoint associated with fibroblast growth factor receptor 1 and centrosomal protein 1. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEP110/CNTRL using anti-CEP110/CNTRL antibody (A07479-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP110/CNTRL antigen affinity purified polyclonal antibody (A07479-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP110/CNTRL at approximately 269 kDa. The expected band size for CEP110/CNTRL is at 269 kDa.
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