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COL3A1 Rabbit Polyclonal Antibody

BP8013
OriGene
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
DownloadMSDS
Product group Antibodies
0.1 ml
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Overview

  • Supplier
    OriGene
  • Product Name
    COL3A1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Protein IDP12105
  • Protein Name
    Collagen alpha-1(III) chain
  • Scientific Description
    COL3A1 rabbit polyclonal antibody, Purified
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.
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