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ProductsBack/Collagen III Polyclonal Antibody
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-Collagen III Polyclonal Antibody (bs-0549R), Unconjugated at 1:800, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-Collagen III Polyclonal Antibody (bs-0549R), Unconjugated at 1:800, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-Collagen III Polyclonal Antibody (bs-0549R), Unconjugated at 1:800, followed by conjugation to the secondary antibody and DAB staining

Collagen III Polyclonal Antibody

BS-0549R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL3A1
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Overview

  • Supplier
    Bioss
  • Product Name
    Collagen III Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:500-2000), IHC-P(1:100-500), IHC-F(1:100-500), IF(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1281
  • Target name
    COL3A1
  • Target description
    collagen type III alpha 1 chain
  • Target synonyms
    EDS4A, EDSVASC, PMGEDSV, collagen alpha-1(III) chain, Ehlers-Danlos syndrome type IV, autosomal dominant, alpha-1 type III collagen, alpha1 (III) collagen, collagen, fetal, collagen, type III, alpha 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02461
  • Protein Name
    Collagen alpha-1(III) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Velázquez KT, Enos RT, Carson MS, et al. miR155 deficiency aggravates high-fat diet-induced adipose tissue fibrosis in male mice. Physiol Rep. 2017,5(18). doi: 10.14814/phy2.13412
    Read this paper
  • Bai Y, Wu C, Hong W, et al. Anti‑fibrotic effect of Sedum sarmentosum Bunge extract in kidneys via the hedgehog signaling pathway. Mol Med Rep. 2017,16(1):737-745. doi: 10.3892/mmr.2017.6628
    Read this paper
  • Yu C, Liu Y, Sun L, et al. Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway. Oncotarget. 2017,8(23):37705-37716. doi: 10.18632/oncotarget.17144
    Read this paper
  • Zhu Y, Hu J, Yu T, et al. High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium. Med Sci Monit. 2016,22:3438-3445.
    Read this paper
  • Zhang F, Dang Y, Li Y, et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. Cell Physiol Biochem. 2016,39(1):294-302. doi: 10.1159/000445624
    Read this paper
  • Yang J, Zhao Q, Wang K, et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. In Vitro Cell Dev Biol Anim. 2016,52(8):846-56. doi: 10.1007/s11626-016-0043-z
    Read this paper
  • Bai Y, Lu H, Lin C, et al. Sonic hedgehog-mediated epithelial-mesenchymal transition in renal tubulointerstitial fibrosis. Int J Mol Med. 2016,37(5):1317-27. doi: 10.3892/ijmm.2016.2546
    Read this paper
  • Jiang K, Chun G, Wang Z, et al. Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes. Mol Med Rep. 2016,13(4):3567-73. doi: 10.3892/mmr.2016.4944
    Read this paper
  • Zhao F, Zhang S, Chen Y, et al. Increased expression of NF-AT3 and NF-AT4 in the atria correlates with procollagen I carboxyl terminal peptide and TGF-β1 levels in serum of patients with atrial fibrillation. BMC Cardiovasc Disord. 2014,14:167. doi: 10.1186/1471-2261-14-167
    Read this paper
  • Su B, Zhao W, Shi B, et al. Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7. Mol Cancer. 2014,13:206. doi: 10.1186/1476-4598-13-206
    Read this paper

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Figure 1. Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.
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