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ProductsBack/Collagen III Polyclonal Antibody
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-Collagen III Polyclonal Antibody (bs-0549R), Unconjugated at 1:800, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-Collagen III Polyclonal Antibody (bs-0549R), Unconjugated at 1:800, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-Collagen III Polyclonal Antibody (bs-0549R), Unconjugated at 1:800, followed by conjugation to the secondary antibody and DAB staining

Collagen III Polyclonal Antibody

BS-0549R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCOL3A1
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Overview

  • Supplier
    Bioss
  • Product Name
    Collagen III Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500), ICC/IF(ICC/IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1281
  • Target name
    COL3A1
  • Target description
    collagen type III alpha 1 chain
  • Target synonyms
    EDS4A, EDSVASC, PMGEDSV, collagen alpha-1(III) chain, Ehlers-Danlos syndrome type IV, autosomal dominant, alpha-1 type III collagen, alpha1 (III) collagen, collagen, fetal, collagen, type III, alpha 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02461
  • Protein Name
    Collagen alpha-1(III) chain
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • miR155 deficiency aggravates high-fat diet-induced adipose tissue fibrosis in male mice. Velazquez KT et al., 2017 Sep, Physiol Rep
    Read this paper
  • Anti-fibrotic effect of Sedum sarmentosum Bunge extract in kidneys via the hedgehog signaling pathway. Bai Y et al., 2017 Jul, Mol Med Rep
    Read this paper
  • Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway. Yu C et al., 2017 Jun 6, Oncotarget
    Read this paper
  • High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium. Zhu Y et al., 2016 Sep 27, Med Sci Monit
    Read this paper
  • Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-beta1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. Zhang F et al., 2016, Cell Physiol Biochem
    Read this paper
  • Isolation and biological characterization of tendon-derived stem cells from fetal bovine. Yang J et al., 2016 Sep, In Vitro Cell Dev Biol Anim
    Read this paper
  • Sonic hedgehog-mediated epithelial-mesenchymal transition in renal tubulointerstitial fibrosis. Bai Y et al., 2016 May, Int J Mol Med
    Read this paper
  • Effect of transforming growth factor-beta3 on the expression of Smad3 and Smad7 in tenocytes. Jiang K et al., 2016 Apr, Mol Med Rep
    Read this paper
  • Increased expression of NF-AT3 and NF-AT4 in the atria correlates with procollagen I carboxyl terminal peptide and TGF-beta1 levels in serum of patients with atrial fibrillation. Zhao F et al., 2014 Nov 25, BMC Cardiovasc Disord
    Read this paper
  • Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7. Su B et al., 2014 Sep 6, Mol Cancer
    Read this paper

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Figure 1. Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.
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