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COL6A2 Rabbit pAb

A3796
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCOL6A2
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Overview

  • Supplier
    ABclonal
  • Product Name
    COL6A2 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1292
  • Target name
    COL6A2
  • Target description
    collagen type VI alpha 2 chain
  • Target synonyms
    BTHLM1; collagen alpha-2(VI) chain; collagen VI, alpha-2 polypeptide; collagen, type VI, alpha 2; epididymis secretory sperm binding protein; human mRNA for collagen VI alpha-2 C-terminal globular domain; PP3610; UCMD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12110
  • Protein Name
    Collagen alpha-2(VI) chain
  • Reactivity Supplier Note
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen VI/COL6A2 antigen affinity purified polyclonal antibody (Catalog # A03194-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen VI/COL6A2 at approximately 150 kDa. The expected band size for Collagen VI/COL6A2 is at 109 kDa.
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