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ProductsBack/Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) Polyclonal Antibody

Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) Polyclonal Antibody

CAU21286
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCSDE1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7812
  • Target name
    CSDE1
  • Target description
    cold shock domain containing E1
  • Target synonyms
    D1S155E, UNR, cold shock domain-containing protein E1, N-ras upstream gene protein, NRAS-related, cold shock domain containing E1, RNA binding, upstream of NRAS
  • Host
    Rabbit
  • Protein IDO75534
  • Protein Name
    Cold shock domain-containing protein E1
  • Scientific Description
    The Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CSDE1/NRU using anti-CSDE1/NRU antibody (A05114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSDE1/NRU antigen affinity purified polyclonal antibody (Catalog # A05114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSDE1/NRU at approximately 89 kDa. The expected band size for CSDE1/NRU is at 89 kDa.
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