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WB analysis of various sample lysates using GTX33106 COMP antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33106 COMP antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33106 COMP antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

COMP antibody

GTX33106
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCOMP
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Overview

  • Supplier
    GeneTex
  • Product Name
    COMP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1311
  • Target name
    COMP
  • Target description
    cartilage oligomeric matrix protein
  • Target synonyms
    CTS2, EDM1, EPD1, MED, PSACH, THBS5, TSP-5, TSP5, cartilage oligomeric matrix protein, cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), multiple epiphyseal dysplasia, pseudoachondroplasia (epiphyseal dysplasia 1, multiple), thrombospondin-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49747
  • Protein Name
    Cartilage oligomeric matrix protein
  • Scientific Description
    The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COMP using anti-COMP antibody (A02443-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat cartilage tissue lysates, Lane 6: mouse cartilage tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMP antigen affinity purified polyclonal antibody (Catalog # A02443-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMP at approximately 110 kDa. The expected band size for COMP is at 83 kDa.
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