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ProductsBack/COMT ELISA Pair
ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 microg/mL and GTX89277 for detection at a concentration of 0.5 microg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 microg/mL and GTX89277 for detection at a concentration of 0.5 microg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 microg/mL and GTX89277 for detection at a concentration of 0.5 microg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.

COMT ELISA Pair

GTX500009
GeneTex
ApplicationsELISA
DownloadDatasheet
Product group Antibodies
TargetCOMT
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Overview

  • Supplier
    GeneTex
  • Product Name
    COMT ELISA Pair - COMT antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 2.5microg/mL and the detection antibody at a concentration of 0.5microg/mL. Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Gene ID1312
  • Target name
    COMT
  • Target description
    catechol-O-methyltransferase
  • Target synonyms
    catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n; HEL-S-98n; testicular tissue protein Li 42
  • Scientific Description
    Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COMT using anti-COMT antibody (PB9539). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody (Catalog # PB9539) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMT at approximately 24 kDa, 28 kDa. The expected band size for COMT is at 30 kDa.
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