WB analysis of membrane enriched extracts (30 ug) of Mouse Brain (Lane 1), NIH/3T3 (Lane 2), PC-3 (Lane 3), C2C12 (Lane 4), HeLa (Lane 5), HCT 116 (Lane 6) and COLO 205 (Lane 7) using GTX22899 COPB antibody. Dilution : 2 microg/ml
WB analysis of membrane enriched extracts (30 ug) of Mouse Brain (Lane 1), NIH/3T3 (Lane 2), PC-3 (Lane 3), C2C12 (Lane 4), HeLa (Lane 5), HCT 116 (Lane 6) and COLO 205 (Lane 7) using GTX22899 COPB antibody. Dilution : 2 microg/ml
COPB antibody
GTX22899
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Hamster, Human, Mouse, Primate, Rat, Yeast
TargetCopb1
Overview
- SupplierGeneTex
- Product NameCOPB antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 2 microg/mL. ICC/IF: 2 microg/mL. FACS: 3-5 microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration4.4 mg/ml
- ConjugateUnconjugated
- Gene ID70349
- Target nameCopb1
- Target descriptioncoatomer protein complex, subunit beta 1
- Target synonyms2610019B04Rik, coatomer subunit beta, beta-COP, beta-coat protein
- HostRabbit
- IsotypeIgG
- Protein IDQ9JIF7
- Protein NameCoatomer subunit beta
- Scientific DescriptionCoatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.
- ReactivityBovine, Hamster, Human, Mouse, Primate, Rat, Yeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Baron DM, Matheny T, Lin YC, et al. Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. Hum Mol Genet. 2019,28(13):2143-2160. doi: 10.1093/hmg/ddz048Read this paper