COPS2 / TRIP15 / ALIEN Antibody

LifeSpan BioSciences

COPS2 / TRIP15 / ALIEN Antibody

23

Western blot analysis of COPS2 expression in A431 (A), Jurkat (B), NIH3T3 (C), mouse brain (D) whole cell lysates. Immunohistochemical analysis of COPS2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of COPS2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.. ICC, IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P, WB (1:500 - 1:2000) Western blot analysis of COPS2 expression in A431 (A), Jurkat (B), NIH3T3 (C), mouse brain (D) whole cell lysates. Immunohistochemical analysis of COPS2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of COPS2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

COPS2

COP9 signalosome subunit 2

ALIEN, CSN2, SGN2, TRIP15, COP9 signalosome complex subunit 2, COP9 constitutive photomorphogenic homolog subunit 2, JAB1-containing signalosome subunit 2, TR-interacting protein 15, TRIP-15, alien homolog, signalosome subunit 2, thyroid receptor-interacting protein 15

Rabbit

-20°C

12352203