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Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated,bs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated,bs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated,bs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

COX4I1 Polyclonal Antibody

BS-10257R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOX4I1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    COX4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1327
  • Target name
    COX4I1
  • Target description
    cytochrome c oxidase subunit 4I1
  • Target synonyms
    COX IV-1; COX4; COX4-1; COXIV; COXIV-1; cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; MC4DN16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13073
  • Protein Name
    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Fine particulate matter induces mitochondrial dysfunction and oxidative stress in human SH-SY5Y cells. Wang Y et al., 2019 Mar, Chemosphere
    Read more
  • Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Kouam AF et al., 2017, Front Pharmacol
    Read more
  • Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol. Wu B et al., 2017 Sep, Biochim Biophys Acta Mol Basis Dis
    Read more
  • De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer. Gao J et al., 2015 Nov, Mol Med Rep
    Read more

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Figure 3. IHC analysis of COX IV using anti-COX IV antibody (A05442-1). COX IV was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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