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COX4I2 Antibody

LS-C812927
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetCOX4I2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    COX4I2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100 - 1:200), IF (1:100 - 1:500), WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84701
  • Target name
    COX4I2
  • Target description
    cytochrome c oxidase subunit 4I2
  • Target synonyms
    COX4, COX4-2, COX4B, COX4L2, COXIV-2, dJ857M17.2, cytochrome c oxidase subunit 4 isoform 2, mitochondrial, COX IV-2, cytochrome c oxidase subunit IV-like 2
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COX4I2 using anti-COX4I2 antibody (A10078-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX4I2 antigen affinity purified polyclonal antibody (Catalog # A10078-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX4I2 at approximately 20 kDa. The expected band size for COX4I2 is at 20 kDa.
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