Bio-Connect
IP analysis of HepG2 cell lysate using GTX33114 COX5B antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000
IP analysis of HepG2 cell lysate using GTX33114 COX5B antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000
IP analysis of HepG2 cell lysate using GTX33114 COX5B antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000

COX5B antibody

GTX33114
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOX5B
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    COX5B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. IHC-P: 1:50 - 1:100. IP: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1329
  • Target name
    COX5B
  • Target description
    cytochrome c oxidase subunit 5B
  • Target synonyms
    COXVB; cytochrome c oxidase polypeptide VB, mitochondrial; cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10606
  • Protein Name
    Cytochrome c oxidase subunit 5B, mitochondrial
  • Scientific Description
    Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetCOX5B
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetCOX5B
  • SizePrice
Product group Antibodies
MedChem Express
TargetCOX5B
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCOX5B
  • SizePrice
Western blot All lanes: COX5B antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 14 kDa Observed band size: 14, 65 kDa
Product group Antibodies
Cusabio
COX5B AntibodyCSB-PA00404A0RB
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetCOX5B
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetCOX5B
  • SizePrice
Anti-COX5B Antibody
Product group Antibodies
Antibodies.com
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetCOX5B
  • SizePrice
Figure 1. Western blot analysis of COX5B using anti-COX5B antibody (A06090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human MCF-7 whole cell lysates. Lane 7: human HCCT tissue lysates, Lane 8: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX5B antigen affinity purified polyclonal antibody (Catalog # A06090-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX5B at approximately 15 kDa. The expected band size for COX5B is at 15 kDa.
Product group Antibodies
Boster Bio
Anti-COX5B Antibody Picoband(r)A06090-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCOX5B
  • SizePrice
Product group Antibodies
References
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCOX5B
  • SizePrice