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CPAMD8 / KIAA1283 Antibody

LS-C782306
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCPAMD8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CPAMD8 / KIAA1283 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Optimal dilution of the CPAMD8 antibody should be determined by the researcher.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (0.1 - 0.5 µg/ml), IHC-P (1 - 2 µg/ml), WB (0.5 - 1 µg/ml) Optimal dilution of the CPAMD8 antibody should be determined by the researcher.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27151
  • Target name
    CPAMD8
  • Target description
    C3 and PZP like alpha-2-macroglobulin domain containing 8
  • Target synonyms
    ASGD8, K-CAP, VIP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8, alpha-2 macroglobulin family protein VIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPAMD8 using anti-CPAMD8 antibody (A12898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPAMD8 antigen affinity purified polyclonal antibody (Catalog # A12898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPAMD8 at approximately 207KD. The expected band size for CPAMD8 is at 207KD.
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