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WB analysis of various sample lysates using GTX33117 CPT2 antibody. Dilution : 1:400 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33117 CPT2 antibody. Dilution : 1:400 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33117 CPT2 antibody. Dilution : 1:400 Loading : 25microg per lane

CPT2 antibody

GTX33117
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCPT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CPT2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1376
  • Target name
    CPT2
  • Target description
    carnitine palmitoyltransferase 2
  • Target synonyms
    CPT1, CPTASE, IIAE4, carnitine O-palmitoyltransferase 2, mitochondrial, CPT II, carnitine palmitoyltransferase II, testicular secretory protein Li 13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23786
  • Protein Name
    Carnitine O-palmitoyltransferase 2, mitochondrial
  • Scientific Description
    The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Liu C, Tate T, Batourina E, et al. Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells. Nat Commun. 2019,10(1):4589. doi: 10.1038/s41467-019-12332-0
    Read this paper
  • You BJ, Hour MJ, Chen LY, et al. Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase. Sci Rep. 2019,9(1):3306. doi: 10.1038/s41598-019-39778-y
    Read this paper
  • You BJ, Chen LY, Hsu PH, et al. Orlistat Displays Antitumor Activity and Enhances the Efficacy of Paclitaxel in Human Hepatoma Hep3B Cells. Chem Res Toxicol. 2019,32(2):255-264. doi: 10.1021/acs.chemrestox.8b00269
    Read this paper

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Figure 1. Western blot analysis of CPT2 using anti-CPT2 antibody (A02112-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT2 antigen affinity purified polyclonal antibody (Catalog # A02112-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT2 at approximately 74KD. The expected band size for CPT2 is at 74KD.
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