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CPT2 Polyclonal Antibody

MBS8567301
MyBioSource
Product group Antibodies
Price on request
0.1 ml
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ISO certified
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Overview

  • Supplier
    MyBioSource
  • Product Name
    CPT2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Estimated Purity
    Affinity Purification
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPT2 using anti-CPT2 antibody (A02112-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT2 antigen affinity purified polyclonal antibody (Catalog # A02112-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT2 at approximately 74KD. The expected band size for CPT2 is at 74KD.
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Lane 1: Hela Cells; Lane 2: 293 Cells; Lane 3: HepG2 Cells; Lane 4: NIH-3T3 Cells; Probed with CPT2 (3B10) Monoclonal Antibody (bsm-52621R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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IHC image of CSB-PA005925LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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