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ProductsBack/CR1 / CD35 Antibody (clone E11, PE, Cy7)

CR1 / CD35 Antibody (clone E11, PE, Cy7)

LS-C812272
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetCR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CR1 / CD35 Antibody (clone E11, PE, Cy7)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E11
  • Conjugate
    Cy7, RPE
  • Estimated Purity
    ...
  • Gene ID1378
  • Target name
    CR1
  • Target description
    complement C3b/C4b receptor 1 (Knops blood group)
  • Target synonyms
    C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement receptor 1; complement receptor type 1; KN; Knops blood group antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD35 using anti-CD35 antibody (A01022-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF1 whole cell lysates, Lane 2: human TF1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD35 antigen affinity purified polyclonal antibody (Catalog # A01022-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD35 at approximately 250 kDa. The expected band size for CD35 is at 224 kDa.
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