More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CRBN Antibody
Western blot All lanes: CRBN antibody at 4.65microg/ml Lane 1: Mouse liver tissue Lane 2: Colo320 whole cell lysate Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western blot All lanes: CRBN antibody at 4.65microg/ml Lane 1: Mouse liver tissue Lane 2: Colo320 whole cell lysate Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western blot All lanes: CRBN antibody at 4.65microg/ml Lane 1: Mouse liver tissue Lane 2: Colo320 whole cell lysate Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa

CRBN Antibody

CSB-PA842761ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCRBN
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    CRBN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51185
  • Target name
    CRBN
  • Target description
    cereblon
  • Target synonyms
    MRT2; MRT2A; protein cereblon; protein x 0001
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SW2
  • Protein Name
    Protein cereblon
  • Scientific Description
    Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-CRBN Antibody144-04722
ApplicationsWestern Blot
TargetCRBN
  • SizePrice
Anti-CRBN Antibody
Product group Antibodies
Antibodies.com
Anti-CRBN AntibodyA14596
ApplicationsWestern Blot
  • SizePrice
Figure 1. Western blot analysis of CRBN using anti-CRBN antibody (A00983-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRBN antigen affinity purified polyclonal antibody (A00983-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRBN at approximately 51 kDa. The expected band size for CRBN is at 51 kDa.
Product group Antibodies
Boster Bio
Anti-CRBN Antibody Picoband(r)A00983-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetCRBN
  • SizePrice
Product group Antibodies
Assay Genie
CRBN AntibodyCAB4722
TargetCRBN
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CRBN / Cereblon AntibodyLS-C682099
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCRBN
  • SizePrice
Product group Antibodies
Bioss Antibodies
CRBN Polyclonal AntibodyBS-11716R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • SizePrice
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Product group Antibodies
Atlas Antibodies
Anti-CRBN AntibodyHPA045910
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCRBN
  • SizePrice
Product group Antibodies
GeneTex
CRBN antibodyGTX131077
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetCRBN
  • SizePrice
Product group Antibodies
Biosynth
CRBN antibody70R-3211
  • SizePrice
Product group Antibodies
Biomatik
Crbn Polyclonal AntibodyCAC10920
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCRBN
  • SizePrice