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ProductsBack/CRBN Antibody
Western blot All lanes: CRBN antibody at 4.65microg/ml Lane 1: Mouse liver tissue Lane 2: Colo320 whole cell lysate Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western blot All lanes: CRBN antibody at 4.65microg/ml Lane 1: Mouse liver tissue Lane 2: Colo320 whole cell lysate Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western blot All lanes: CRBN antibody at 4.65microg/ml Lane 1: Mouse liver tissue Lane 2: Colo320 whole cell lysate Lane 3: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa

CRBN Antibody

CSB-PA842761ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCRBN
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Overview

  • Supplier
    Cusabio
  • Product Name
    CRBN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51185
  • Target name
    CRBN
  • Target description
    cereblon
  • Target synonyms
    MRT2; MRT2A; protein cereblon; protein x 0001
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SW2
  • Protein Name
    Protein cereblon
  • Scientific Description
    Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CRBN using anti-CRBN antibody (A00983-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRBN antigen affinity purified polyclonal antibody (A00983-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRBN at approximately 51 kDa. The expected band size for CRBN is at 51 kDa.
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