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Formalin-fixed and paraffin embedded human cervial carcinoma labeled with Anti-CREB-1 Polyclonal Antibody, Unconjugated (bs-0035R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human cervial carcinoma labeled with Anti-CREB-1 Polyclonal Antibody, Unconjugated (bs-0035R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human cervial carcinoma labeled with Anti-CREB-1 Polyclonal Antibody, Unconjugated (bs-0035R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

CREB-1 Polyclonal Antibody

BS-0035R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCREB1
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Overview

  • Supplier
    Bioss
  • Product Name
    CREB-1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1385
  • Target name
    CREB1
  • Target description
    cAMP responsive element binding protein 1
  • Target synonyms
    CREB, CREB-1, cyclic AMP-responsive element-binding protein 1, active transcription factor CREB, cAMP-response element-binding protein-1, cyclic adenosine 3',5'-monophosphate response element binding protein, cyclic adenosine 3',5'-monophosphate response element-binding protein CREB, transactivator protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16220
  • Protein Name
    Cyclic AMP-responsive element-binding protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang N, Wen Q, Ren L, et al. Neuroprotective effect of arctigenin via upregulation of P-CREB in mouse primary neurons and human SH-SY5Y neuroblastoma cells. Int J Mol Sci. 2013,14(9):18657-69. doi: 10.3390/ijms140918657
    Read this paper

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Figure 1. Western blot analysis of CREB1 using anti-CREB1 antibody (A00577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antigen affinity purified polyclonal antibody (Catalog # A00577-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB1 at approximately 43,49 kDa. The expected band size for CREB1 is at 36,37,25 kDa.
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