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Creb3L1 Polyclonal Antibody

CAC11054
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetCREB3L1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Creb3L1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:200-1:5000, IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID90993
  • Target name
    CREB3L1
  • Target description
    cAMP responsive element binding protein 3 like 1
  • Target synonyms
    C16DELp11.2, DEL16p11.2, OASIS, OI16, cyclic AMP-responsive element-binding protein 3-like protein 1, BBF-2 homolog, Osteogenesis imperfecta, type XVI, cAMP-responsive element-binding protein 3-like protein 1, old astrocyte specifically-induced substance
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96BA8
  • Protein Name
    Cyclic AMP-responsive element-binding protein 3-like protein 1
  • Scientific Description
    The Creb3L1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CREB3L1 using anti-CREB3L1 antibody (PB9492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: MM231 Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L1 antigen affinity purified polyclonal antibody (Catalog # PB9492) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB3L1 at approximately 57 kDa. The expected band size for CREB3L1 is at 57 kDa.
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