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Western Blot analysis of HeLa cells using CREB3L2 Polyclonal Antibody
Western Blot analysis of HeLa cells using CREB3L2 Polyclonal Antibody
Western Blot analysis of HeLa cells using CREB3L2 Polyclonal Antibody

CREB3L2 Antibody

CSB-PA001784
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCREB3L2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CREB3L2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64764
  • Target name
    CREB3L2
  • Target description
    cAMP responsive element binding protein 3 like 2
  • Target synonyms
    basic transcription factor 2; BBF2 human homolog on chromosome 7; BBF2H7; B-ZIB transcription factor; cAMP-responsive element-binding protein 3-like protein 2; cyclic AMP-responsive element-binding protein 3-like protein 2; FUS/BBF2H7 protein; TCAG_1951439
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ70SY1
  • Protein Name
    Cyclic AMP-responsive element-binding protein 3-like protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CREB3L2 using anti-CREB3L2 antibody (A04591-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L2 antigen affinity purified polyclonal antibody (A04591-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB3L2 at approximately 60 kDa. The expected band size for CREB3L2 is at 57 kDa.
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